Journal: PLoS ONE

Article Title: Hearts of Dystonia musculorum Mice Display Normal Morphological and Histological Features but Show Signs of Cardiac Stress

doi: 10.1371/journal.pone.0009465

Figure Lengend Snippet: Primers used for QPCR experiments.

Article Snippet: Quantitative polymerase chain reaction (QPCR) (MJ Research) was performed in triplicate for each sample using primer pairs as detailed in .

Techniques:

Journal: PLoS ONE

Article Title: Hearts of Dystonia musculorum Mice Display Normal Morphological and Histological Features but Show Signs of Cardiac Stress

doi: 10.1371/journal.pone.0009465

Figure Lengend Snippet: mRNA was isolated from wt and dt hearts and QPCR was performed to assess transcript levels of other plakin proteins expressed in cardiac tissue.

Article Snippet: Quantitative polymerase chain reaction (QPCR) (MJ Research) was performed in triplicate for each sample using primer pairs as detailed in .

Techniques: Isolation

Journal: Frontiers in Immunology

Article Title: Elevated Expression of miR-19b Enhances CD8 + T Cell Function by Targeting PTEN in HIV Infected Long Term Non-progressors With Sustained Viral Suppression

doi: 10.3389/fimmu.2018.03140

Figure Lengend Snippet: miRNA profiles distinguish LTNPs with different levels of virus. (A) Principal component analysis (PCA) plot of miRNA expression data from LTNPs, TPs, and HCs in the training cohort. Nine LTNPs were divided into two groups, one of which was very close to the TPs (Group A, n = 6) and another that was intertwined with the HCs (Group B, n = 3). (B) Comparison of age, CD4 counts, and viral load between Group A and Group B of LTNPs. * P < 0.05.

Article Snippet: Quantitative real-time polymerase chain reaction (qRT-PCR)-based high-throughput miRNA profiling was performed at QuantoBio Biotechnology Co. Ltd. (Beijing, China).

Techniques: Virus, Expressing, Comparison

Journal: Frontiers in Immunology

Article Title: Elevated Expression of miR-19b Enhances CD8 + T Cell Function by Targeting PTEN in HIV Infected Long Term Non-progressors With Sustained Viral Suppression

doi: 10.3389/fimmu.2018.03140

Figure Lengend Snippet: Expression of miR-19b is high in LTNP-Ls compared with that observed in LTNP-Hs. (A) Heatmap demonstrating 78 miRNAs differentially expressed between LTNP-Hs ( n = 6) and LTNP-Ls ( n = 3) in the training cohort (Benjamini–Hochberg false discovery rate-adjusted P < 0.05 and fold change >2). Hierarchical clustering of change in the threshold cycle (ΔCT) was performed using the complete linkage method and Pearson correlation coefficient. (B) The protocol for the selection of candidate miRNA from the training cohort. Among the 78 miRNAs differentially expressed between LTNP-Hs ( n = 6) and LTNP-Ls ( n = 3), 70 miRNAs differentially expressed between LTNPs and HCs ( P < 0.05) were excluded. Subsequently, five differentially expressed miRNAs between LTNPs and TPs were excluded. Three candidate miRNAs, namely miR-15a, miR-19b, and miR-33 were selected. (C) Comparison of the three candidate miRNAs between LTNP-Ls ( n = 3) and LTNP-Hs ( n = 6) in the training cohort. (D,F) Relative expression of miR-19b (D) , miR-15a (E) and miR-33 (F) in PBMCs obtained from LTNP-Ls ( n = 8) and LTNP-Hs ( n = 10) in the subsequent validation group. (G,H) CD4 + and CD8 + T cells from LTNPs were sorted through flow cytometry. The expression of miR19b in CD4 + (G) and CD8 + T (H) cells was compared between LTNP-Ls ( n = 9, one from training cohort, eight from validation cohort) and LTNP-Hs ( n = 10, two from training cohort, eight from validation cohort) using qRT-PCR. * P < 0.05.

Article Snippet: Quantitative real-time polymerase chain reaction (qRT-PCR)-based high-throughput miRNA profiling was performed at QuantoBio Biotechnology Co. Ltd. (Beijing, China).

Techniques: Expressing, Selection, Comparison, Biomarker Discovery, Flow Cytometry, Quantitative RT-PCR